Decyder 2d software user manual

2D Electrophoresis, Principles and Methods, GE Healthcare. DeCyder 2D software version user manual, GE Healthcare. Ettan DIGE System, User Manual, GE Healthcare. Standard 2D DIGE procedure (User Manual ) Create pick list (DeCyder software) Picking spots of interest (Ettan spot picker) Trypsin digestion (Ettan digester) Spotting (Ettan spotter) Masspectrometry (Ettan MALDI ToF Pro) Identify proteins of interest 1 6 20°C 37°C RuvB-Cy3 His-Cy5 t 0 t 1t 2 t 3 t 4 t 0 t t 2 t 3 t 4 RuvB His-GFP 20°C 37°C. software. DeCyder™ 2-D Differential Analysis Software (DeCyder 2D) v is specifically designed for 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) analysis and is a key element in the DIGE system. Unlike other 2-D electrophoresis methods, DIGE allows the option of reference to an internal standard for each spot, effectively.

DeCyder 2-D software v start-up screen showing analysis workflow. DeCyder 2-D software is unique in fully exploiting the advantages offered by multiplexing, the co-migration of more than one sample per gel, by allowing the inclusion of an internal standard. Multiplexing is performed using proprietary size- and. Standard 2D DIGE procedure (User Manual ) Create pick list (DeCyder software) Picking spots of interest (Ettan spot picker) Trypsin digestion (Ettan digester) Spotting (Ettan spotter) Masspectrometry (Ettan MALDI ToF Pro) Identify proteins of interest 1 6 20°C 37°C RuvB-Cy3 His-Cy5 t 0 t 1t 2 t 3 t 4 t 0 t t 2 t 3 t 4 RuvB His-GFP 20°C 37°C. Typhoon FLA DeCyder™ 2D software v Ettan Spot Picker Ettan Digester ECL Plex™ ImageQuant TL with ImageQuant TL SecurITy Sample preparation Protein separation Blotting Antibody probing Detection Image acquisition Image analysis SDS-PAGE Clean-Up Kit MiniVE SE /SE SE Ruby EPS /EPS Rainbow™ Markers ECL™ DualVue.

Differential Analysis Software designed for use in 2-D Fluorescence Difference Gel Electrophoresis. DeCyder™ 2-D Differential Analysis Software has been. This release of DeCyder version 7 (software) is provided by GE system can be found in the Ettan DIGE System User Manual (code. ). the availability of competing technologies and the widespread use of 2D PAGE in various GE's Typhoon Trio imager with DeCyder 2D analysis software.